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U-CUP

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The U-CUP perfusion bioreactor is a user-friendly tool to establish and control your 3D cell and tissue cultures.


U-CUP has been specifically designed to be used by any scientist or lab technician working in the life science related field, without necessarily requiring any previous experience with the use of bioreactors.

Applications
  • Organotypic models (Bone remodeling, Tumor microenvironment)
  • 3D cell expansion and differentiation  
  • Investigation of cell-scaffold interactions  
  • Investigation of cell-extracellular matrix interactions  
  • Generation of 3D cell-scaffold constructs suitable for preclinical experimentation

Apply instantly your current cell culture concepts and simply let U-CUP further extend them by performing the seamless transition to the 3D context.

FeaturesAdvantagesBenefits
Direct perfusionUnifrom cell seeding Uniform tissue  
Efficient nutrition and waste removal Viable tissue, up to several weeks of culture 
Physiological conditions (mimicking inetrstitial fluid flow and associated induced shearsPhysiologically relevant tissue
Simple and smart design (patented)Easy and ready to useNo previous experience with 3D cell cultures required 
Minimized manual operationsHighly reproducible results 
Efficient with many cell typesVersatile cell and tissue culture models 
Supple scaffold adaptorsCompatible with a wide spectrum of 3D porous scaffolds of various composition, architecture and sizes 
Access to cell culture medium through valvesSuitable to seed and co-culture several cell types, even at different culture time points  Possibility to investigate complex cell-cell and cell-extracellular matrix interaction 
Efficient cell retrieval from scaffolds after culture (with standard enzymatic treatment) Easy cell analyses (cytofluorimetry, gene expression etc.) 


Product Configuration

1x syringe pump

 

1x rack

 10x U-CUP disposable bioreactor kits 

1x Starter kit

++=
 The driving force of the system. It generates the oscillating fluid flow of the cell/medium suspension. It cannot be purchased separately. A rotating rack for easy and correct positioning of U-CUP disposable bioreactors. It can also be purchased separately. The central core of the system. It is disposable and it comes with 10x adaptors to fit the specific size of your scaffolds. Scaffolds can also be purchased separately. It provides all what you need to start your 3D cell cultures. Additional accessories (e.g. forceps, syringes) and testing units are also included.

The performance of U-CUP has been extensively validated and certified by scientific publications in peer-review journals.

If you are convinced of the benefits that a 3D culture environment can provide,the U-CUP bioreactor is the essential tool to conduct with your experiments.

For assistance and advice to set up your experiment, do not hesitate to contact CELLEC’s expert team to address your questions.

REPAIRLab, University Medical Center, Mainz, Germany

We have been using the U-CUP perfusion bioreactor for approximately 1 year. Due to excellent technical assistance, detailed protocols and a hands-on introduction at the facilities, only a short time was needed to get everything up and running in our lab.  We have carried out studies for up to 28 days with primary cells.  The system takes up little space, is easily handled and manipulated ensuring sterility and the results are highly reproducible between multiple samples. The team at CellecBiotek AG, especially Ms. Anna Gril and Dr. Adam Papadimitropoulos have provided professional guidance before and after the purchase, as well as well as excellent scientific support. It is not a matter of course that critical questions are discussed and that scientific details are openly shared – and for this we are very grateful!

Dr. Thomas Böse
REPAIRLab, University Medical Center
Johannes Gutenberg University Mainz
Mainz, GERMANY

We work on hematopoietic niches, more precisely on the interactions between hematopoietic stem cells and their microenvironment. We had previously developed an in vitro 3D niche composed of different bone marrow stromal cells and demonstrate the functionality of our model by its capacity to maintain or promote stemness/quiescence of hematopoietic stem cells. We wanted to increment our system by adding flow to better fit with the bone marrow physiology. We have purchased the U-CUP perfusion bioreactor from CELLEC. We really see that CELLEC proposes a “ready-to-use” system. 

However, despite the very user-friendly side of the U-CUP, we faced several difficulties to have a good flow and to correctly colonize our biomaterial. We wrote to the scientific expert to have advices and each time we had a quick and powerful answer adapted to our problems. They really perfectly know their product and have a large scientific culture to bring adapted solutions.

The U-CUP from Cellec fulfilled our wishes and, even more, open to us new research fields!


Adrienne Anginot, PhD

INSERM U972 Institut André Lwoff

Université Paris XI, Hôpital Paul Brousse

Villejuif, FRANCE

Related Files

U-CUP flyer Poster

By adding the third dimension to cells environment one attempts to approach the milieu of the most biological tissues and organs found in nature, which are organised in 3D and to recapitulate the complex cellular interactions encountered therein. Therefore, 3D cell culture-based models are expected to capture similar aspects of those observed in vivo, leading thus to more relevant scientific results. In creating 3D cell culture models, U-CUP becomes an essential part of this process by both simplifying the procedure itself and generating solid scientific data.

Specifications:

System Size 

 Scaffold type

Scaffold size  

Working volume

Perfusion velocity 

Cell density 

 Cell type

Rack hosts up to 10 independent U-CUP bioreactors and fits into an incubator

 Rigid or soft; ceramic, synthetic or natural polymer based

Thickness:      
2 – 4 mm
Diameter:
6 – 8 – 10 mm

 6 to 14 ml per bioreactor

 1 μm/s to 10000 μm/s (or higher if required)

 Wide range (up to tens of millions per bioreactor)

freshly isolated, expanded cells, cell lines



How does it work?

 

Options for cell or tissue culture in the U-CUP bioreactor
  • Cell retrieval: Cells can be efficiently retrieved thanks to the perfusion flow by enzymatic digestion and then analyzed with typical lab equipment (e.g. RT-PCR, FACS)
  • Tissue harvesting: Tissue constructs are employed as such for in vitro analyses (e.g. histology) or pre-clinical use (e.g. in vivo implantation)
Porous scaffolds, providing a 3D template for cells to settle and grow or develop a tissue, are also available through our website

How can I assemble the U-CUP bioreactor?

  • Muraro and Muenst 2017 presents an application for ex-vivo culture of human breast cancer specimens (Title: Ex-vivo assessment of drug response on breast cancer primary tissue with preserved microenvironments, OncoImmunology)
  • Boccardo and Gaudiello 2016 In this paper, the perfusion-based bioreactor is used for the generation of an adipose mesenchymal stromal cells -based engineered constructs (Title: Engineered mesenchymal cell-based patches as controlled VEGF delivery systems to induce extrinsic angiogenesis, Acta Biomaterials)
  • Cerino 2016 presents an application for engineering an in vitro 3D multi-cellular muscle-like tissue model (Title: Three-dimensional multi-cellular muscle-like tissue engineering in perfusion-based bioreactors, Biotechnology and Bioengineering)
  • Hirt and Papadimitropoulos 2015 demonstrates the importance of perfusion flow in 3D cultures of tumor cells to efficiently mimic functional features observed “in vivo” and to test anticancer compounds (Title: Bioreactor-engineered cancer tissue-like structures mimic phenotypes, gene expression profiles and drug resistance patterns observed in vivo, Biomaterials)
  • Centola 2015 In this study, the perfusion-based bioreactor system is used to improve cartilage digestion, resulting in higher and more reproducible yield of cell populations with high proliferation and chondrogenic capacity (Title: An improved cartilage digestion method for research and clinical applications, Tissue Engineering Part C, Methods)
  • Bao 2015 presents a humanized in vitro model that reduces the need for experimental animal models, while recapitulating key biological events in a periodontal pocket (Title: Establishment of an oral infection model resembling the periodontal pocket in a perfusion bioreactor system, Virulence)
  • Papadimitropoulos 2014 presents an efficient expansion method of mesenchymal stromal cells by direct seeding and culturing fresh bone marrow preparation within the pores of 3D porous scaffold (Title: Expansion of human mesenchymal stromal cells from fresh bone marrow in a 3D scaffold-based system under direct perfusion, PLoS One)
  • Hirt 2014 highlights the potential of perfusion-based models to create 3D tumour microenvironment for cancer immunobiology studies and pre-clinical assessment of innovative treatments (Title: In vitro 3D models of tumor-immune system interaction, Advance Drug Delivery Review)
  • Papadimitropoulos 2013 presents an application/method for seeding open porous rapid prototyped polymeric scaffolds (Title: A collagen network phase improves cell seeding of open-pore structure scaffolds under perfusion, Journal of Tissue Engineering and Regenerative Medicine)
  • Sadr 2012 presents an application/method to generate a decellularized cell-laid extacellular matrix which enhances the biological performance of polymeric materials (Title: Enhancing the biological performance of synthetic polymeric materials by decoration with engineered, decellularized extracellular matrix, Biomaterials)
  • Gueven 2011 presents an application for upscaling osteogenic and vasculogenic grafts (Title: Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue, Biomaterials)
  • Papadimitropoulos 2011 presents an application for engineering an in vitro bone organ model (Title: A 3D in vitro bone organ model using human progenitor cells, European Cell & Materials)
  • Di Maggio 2011 a review for our approaches to engineering in 3D vitro niches (Title: Toward modeling the bone marrow niche using scaffold-based 3D culture systems, Biomaterials)
  • Santoro 2010 presents an application for upscaling cartilaginous grafts (Title: Bioreactor based engineering of large-scale human cartilage grafts for joint resurfacing, Biomaterials)
  • Scherberich 2007 presents an application for generating osteogenic and vasculogenic grafts (Title: Three-dimensional perfusion culture of human adipose tissue-derived endothelial and osteoblastic progenitors generates osteogenic constructs with intrinsic vascularization capacity, Stem Cells)
  • Wendt 2006 describes the system for maintaining living uniform tissues in the scaffolds (Title: Uniform tissues engineered by seeding and culturing cells in 3D scaffolds under perfusion at defined oxygen tensions, Biorheology)
  • Braccini 2005 presents an application for generating osteogenic grafts (Title: Three-dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts, Stem Cells)
  • Wendt 2003 describes the principle of the U-CUP and its impact on cell seeding (Title: Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity, Biotechnology and Bioengineering)


 
 
 
 

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